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KMID : 0385220000100030022
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Korean Journal of Gerontology
2000 Volume.10 No. 3 p.22 ~ p.31
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Effects of Squalene in the Skin af Aged Mouse
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Choi Wan-Soo
Kim Jong-Se
Kim Se-Jong
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Abstract
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To evaluate the effect of squalene (SQ) on the skin of the aged mouse, SQ was applied to the skin on the back of 10 month-aged ICR female mouse at 12 hour-interval for 10 days. Biochemical quantitative analysis of total protein, the expression of epidermal growth factor (EGF) and histologic alterations in the SQ-treated skin using light and laser confocal microscopes were performed. Total protein quantity was increased with the period of SQ treatment in SQ-treated group rather than in normal aged control one. The increment was especially remarkable on protein with 42 kDa. In laser confocal in croscopic observation, the expression of EGF was increased with time after 24 hours of SQ treatment whereas no expression was observed in normal aged skin. In light microscopic oservation, the development of germ cell layer was weak in normal aged skin, the number of the cells, however, started to increase after 2 days and the differentiation of germ cell layer was remarkable after 3 days of SQ treaInment. Time increment of the number of epidermal cells was directly proportional to the period of SQ treatment and the whole cell layers in epidermis were easily differentiated after 5 days of SQ treatment. The application of SQ to the skin of aged mouse induced the increase of total protein and expression of EGF, and germ cell differentiation. Therefore, it is suggested that SQ plays a major role in differentiation or renewal of skin tissue of aged mouse. f Key words: Squalene, Epidermal growth factor, Laser confiuc뭓l microscopic, Aged mouse
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KEYWORD
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Squalene, Epidermal growth factor, Laser confocal microscopic, Aged mouse
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